Monoclonal antibodies Atg5-7C6 and Atg5-11C3 for the detection of endogenous Atg5 in immunoblotting, immunocytochemistry as well as in flow cytometry.
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For further product information please click here:
0262-100/ATG5-7C6
0263-100/ATG5-11C3
Monoclonal antibody LC3-5H3 for the detection of endogenous LC3A, B, C (microtubule-associated
protein 1 light chain 3 A,B,C) in immunoblotting.
For detailed product information please click here: 0261-100/LC3-5H3
Monoclonal antibody Beclin-12B4 for the detection of endogenous Beclin in immunoblotting.
For further information please click here: 0240-100/Beclin-12B4
New improved Anti-LC3 Monoclonal antibody for Western-Blot Applications
For further information please click here : 0260-100/LC3-2G6
Monoclonal antibody LC3-5F10
for the detection of endogenous
LC3B (microtubule-associated
protein 1 light chain 3B) in immunocytochemistry (see images below) as well as in immunoblotting.
For immunocytochemistry please see also latest citation: J.Biol. Chem., 282 (33),24131-24145, August 17, 2007.
0231-100/LC3-5F10
(A) (B)
Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine-stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10 (Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).
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